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PPP, buffy coat, and e-PRF induced a substantial increase of <t>IL11</t> in gingival fibroblasts, showing expression as x-fold increases compared with untreated cells. ( A ) Real-time PCR analysis of IL11. The expression level of the untreated cells was set to 1 for calibration. ( B ) Quantification of IL11 levels in the supernatant by immunoassay. Statistical analysis was based on the ratio-paired t -test. Wo stood for the untreated cells. N = 4, and different symbol shapes indicate independent experiments. Significance was set at p < 0.05.
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PPP, buffy coat, and e-PRF induced a substantial increase of IL11 in gingival fibroblasts, showing expression as x-fold increases compared with untreated cells. ( A ) Real-time PCR analysis of IL11. The expression level of the untreated cells was set to 1 for calibration. ( B ) Quantification of IL11 levels in the supernatant by immunoassay. Statistical analysis was based on the ratio-paired t -test. Wo stood for the untreated cells. N = 4, and different symbol shapes indicate independent experiments. Significance was set at p < 0.05.

Journal: International Journal of Molecular Sciences

Article Title: Extended PRF: Impact of Heat on Gene Expression in Gingival Fibroblasts

doi: 10.3390/ijms26189120

Figure Lengend Snippet: PPP, buffy coat, and e-PRF induced a substantial increase of IL11 in gingival fibroblasts, showing expression as x-fold increases compared with untreated cells. ( A ) Real-time PCR analysis of IL11. The expression level of the untreated cells was set to 1 for calibration. ( B ) Quantification of IL11 levels in the supernatant by immunoassay. Statistical analysis was based on the ratio-paired t -test. Wo stood for the untreated cells. N = 4, and different symbol shapes indicate independent experiments. Significance was set at p < 0.05.

Article Snippet: The immunoassay for human IL11 (DY218, R&D Systems, Minneapolis, MN, USA) was performed with the supernatant of gingival fibroblasts exposed to the lysates of PPP, heated PPP (Alb-gel), buffy coat (BC), and e-PRF.

Techniques: Expressing, Real-time Polymerase Chain Reaction